In vitro Cosmetics
DEMONSTRATE THE EFFICACY OF YOUR FINAL PRODUCT OR INGREDIENT
Protection from UV / IR / HEV – induced damage
We analyse whether an ingredient or final product protects against the oxidative stress, aging and senescence induced by ultraviolet (UV), infrared (IR) or blue light (high-energy visible, HEV) radiation, quantifying the reactive oxygen species (ROS) generated, the cell viability decrease or the cells health.
DNA protection and repair from UV damage
We analyse whether an ingredient or final product protects against DNA lesions provoked by UV-B radiation, through quantification of thymine dimers by flow cytometry. At the same time, we determine the capacity of after-sun products to improve repair of damages induced by UV-B radiation.
Antioxidant and Anti-aging
We determine the antioxidant capacity of a compound through quantification of cellular metabolites (GSSH, GSSG, Ophthalmic Acid, 2dG, 8OHdG, etc.) involved in the antioxidant protection system, by HPLC-MS/MS. Moreover, we quantify the expression of different genes involved with aging and the antioxidant system (Telomerase, SOD, Catalase, GPx, Nrf2, Oct4, etc.).
Using confocal microscope, we analyze the capacity of a product to penetrate into the cutaneous surface. We use Human Reconstructed 3D skin or Pig skin and we can determine whether a vehicle (e.g lyposomes) improve penetration, as well as compare different products or vehicles among them.
Firming: Collagen, Elastin and MMPs
We analyze the capacity of a product or ingredient to stimulate or inhibit collagen synthesis, elastin, matrix metalloproteinases (MMPs), fibronectin, laminin, etc. both at gene (RT-qPCR) and protein (ELISA) levels.
Studies may be performed on Human 3D Reconstructed Skin (image).
Cytotoxicity and Regeneration
We use different assays to analyze the proliferative and regenerative capacity of a product or ingredient, upon various cellular and embryonic platforms. Moreover, these studies allow us to determine the effective concentration of a compound, optimizing production. The MTT and Streak (Wound-healing) assays are the most usual ones.
Confocal Microscopy (Human 3D Skin)
We analyze using confocal microscopy through the use of stains and specific antibodies, biomarkers such us cell proliferation (PCNA), oxidative stress (NRF2), apoptosis, hematoxilin-eosin staining, phalloidin staining, etc; upon human cell lines, pigskin and RHE (Reconstructed Human 3D Epidermis).
Anti-Hair loss – Inhibition of 5-alpha reductase
It is widely demonstrated that enzyme 5-alpha reductase is involved in the development of androgenic alopecia, which constitutes 95 % of alopecia cases. The inhibition of this enzyme is the target of multiple compounds in the market from two decades ago, including Finasteride and Minoxidil.
At Bionos, we analyze in vitro the capacity of a compound to inhibit the expression of 5-alpha reductase isoforms, upon dermal papilla cells from hair follicle.