In vitro Cosmetics
DEMONSTRATE THE EFFICACY OF YOUR FINAL PRODUCT OR INGREDIENT
DNA protection and repair from UV damage
We analyse whether an ingredient or final product protects against DNA lesions provoked by UV-B radiation, through quantification of thymine dimers by flow cytometry. At the same time, we determine the capacity of after-sun products to improve repair of damages induced by UV-B radiation.
Antioxidant and Anti-aging
We determine the antioxidant capacity of a compound through quantification of cellular metabolites (GSSH, GSSG, Ophthalmic Acid, 2dG, 8OHdG, etc.) involved in the antioxidant protection system, by HPLC-MS/MS. Moreover, we quantify the expression of different genes involved with aging and the antioxidant system (Telomerase, SOD, Catalase, GPx, Nrf2, Oct4, Nanog, Wnt, etc.).
Through the confocal microscope, we analyse the capacity of a product to penetrate into the cutaneous surface. We use human reconstituted 3D skin or pig skin and we can determine whether a vehicle (e.g lyposomes) improve penetration, as well as compare different products or vehicles between them.
Cytotoxicity and Regeneration
We develop different assays to analyse the proliferative and regenerative capacity of a product or ingredient, upon various cellular and embryonic platforms. Moreover, these studies allow us to determine the effective concentration of a compound, optimising production. The MTT and Streak (Wound-healing) assays are the most usual ones.
Confocal Microscopy (Human 3D Skin)
We analyse by confocal microscopy through the use of stains and specific antibodies, biomarkers such us cell proliferation (PCNA), oxidative stress (NRF2), apoptosis, hematoxilin-eosin staining, phalloidin staining, etc; upon human cell lines, pigskin and RHE (Reconstituted Human 3D Epidermis).
Anti-Hair loss – Inhibition of 5-alpha reductase
It is widely demonstrated that enzyme 5-alpha reductase is involved in the development of androgenic alopecia, which constitutes 95 % of alopecia cases. The inhibition of this enzyme is the target of multiple compounds in the market from two decades ago, including Finasteride.
At Bionos, we in vitro analyse the capacity of a compound to inhibit the expression of 5-alpha reductase, upon dermal papilla cells from hair follicle.