Cosmetics in vitro

Demonstrate the Efficacy of your Final Product or Ingredient

 

Protection from UV / IR / HEV – induced damage

We analyze whether an ingredient or final product protects against the oxidative stress, aging and senescence induced by ultraviolet (UV), infrared (IR) or blue light (high-energy visible, HEV) radiation, quantifying the reactive oxygen species (ROS) generated or cell viability decrease.

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF, human follicle dermal papilla cells HFDPC).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).

 

 

Anti-pollution – Oxidative Stress

We assess the protective capacity of a determined product against the oxidative stress induced by urban pollution, through quantification of reactive oxygen species (ROS) or cell viability decrease, after damage induction with dust particles obtained from urban environment (Urban Dust).

  • Keratinocytes HaCaT and NHEK.
  • Fibroblasts NHDF.
  • Human Follicle Dermal Papilla Cells (HFDPC).
  • 3D Reconstructed Human Skin (RHE).

 

 

Whitening / Tanning

We analyze the capacity of an ingredient or end product to inhibit (whiten) or stimulate (self-tan) melanin synthesis, through quantification of melanin and the expression of genes involved in melanogenesis.

  • Human cell lines (epidermal melanocytes NHEM).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).
  • Mouse cell lines (melanocytes B16).

 

 

Confocal Microscopy – Penetration and Imaging

Through the use of confocal microscopy, we assess the capacity of a compound to penetrate into cutaneuos surface, as well as its efficacy and functionality by imaging techniques; using stainings (DAPI, hematoxylin-eosin, phalloidin, PCNA…) and specific antibodies (NRF2, ROS, apoptosis…).

  • Pig skin (sus scrofa domestica) recently obtained.
  • 3D Reconstructed Human Skin (RHE).

 

 

Inflammaging – Anti-irritation and Calming effect

We evaluate the capacity of a compound to protect against the inflammatory response induced by an external agent such as ultraviolet radiation (UV) or Bacterial Lipopolysaccharide (LPS), quantifying biomarkers involved in inflammation and immunity like TNFalpha, IL-1a, IL-1b, IL-6, IL-8, IL-10, NFkB, TGFb, etc.

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF).
  • Peripheral Blood Mononuclear Cells from human volunteers (PBMC).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).

 

 

Antioxidant and Antiaging

We determine the antioxidant capacity through quantification of intracellular metabolites (GSH, GSSG, Opthalmic Acid, 2dG, 8OHdG, etc.) involved in antioxidant protection system, by UPLC-MS/MS. Likewise, we quantify the capacity of a product to stimulate the expression of genes involved in aging and antioxidant system (Tert, SOD1, SOD2 Cat, GPx1, Nrf2, Oct4, Nanog, Wnt, etc.).

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF, human follicle dermal papilla cells HFDPC).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).

 

 

Firming: Extracellular Matrix

We assess the capacity of a final cosmetic product or active ingredient to stimulate or inhibit the synthesis of collagen, elastin, matrix metalloproteinases (MMPs), fibronectin, lamimin, versican, lumican, etc.. both at genetic level through RT-qPCR and at protein level through ELISA.

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).

 

 

Proliferation and Regeneration

We develop different assays to analyse the proliferative and regenerative capacity of a product or functional ingredient, on cellular platforms, through quantification of cell viability (MTT assay) and image quantification (Wound-Healing). At the same time, these assays allow us to determine the cytotoxicity of a determined product, as well as the effective concentration to be used in subsequent assays.

  • Keratinocytes HaCaT and NHEK.
  • Fibroblasts NHDF.
  • Human Follicle Dermal Papilla Cells (HFDPC).
  • Melanocytes NHEM.

 

Moisturizing and Antiaging: Hyaluronic Acid

We assess the capacity of a determined product or active ingredient to stimulate the synthesis of hyaluronic acid (HA), helping to detoxify skin and delaying the onset of aging markers. Protein quantification by ELISA and gene expression by RT-qPCR (hyaluronic acid, aquaporines, hyaluronan synthases, hyaluronidases…).

  • Keratinocytes HaCaT and NHEK.
  • Fibroblasts NHDF.

 

 

UVB-induced DNA Protection and Repair

We evaluate whether a determined ingredient or end product protects from the DNA lesions caused in the DNA by the UVB radiation, through quantification of thymine dimers (T-T) through flow cytometry. In the same way, we assess the capacity of post-sun products to improve endogenous DNA repair of UVB-induced damages.

 

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF, human follicle dermal papilla cells HFDPC).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).

 

 

Anti-hair loss – 5a-Reductase Inhibition

It is widely demonstrated that 5a-Reductase enzyme is involved in the development of androgenetic alopecia, which constitutes 95 % of hair loss cases. The inhibition of this enzyme is the goal of several anti-hair loss treatments in the market since more than 20 years ago. We analyze in vitro the capacity of a determined compound to inhibit the expression or synthesis of 5a-Reductasa, through quantification by ELISA or RT-qPCR.

  • Human Follicle Dermal Papilla Cells (HFDPC).
  • Keratinocytes HaCaT and NHEK.
  • Medaka eleutheroembryos.

 

Hair Proliferation – Philpott Test

According to the culture conditions established by Philpott et al., 1990, we determine the capacity of a treatment to stimulate the growth of hair follicles directly extracted from human volunteers. This way, we obtain data from hair proliferation stimulation, through comparison with the untreated basal control.

Pictures from microscopy are included for each of the tested conditions.

  • Hair Follicles extracted from human volunteers.

 

Thermal Shock Protection (HSPs)

Thermal stress may damage cells, both through proliferation and viability levels and morphology and structural. Heat Shock Proteins (HSPs) are a group of defensive proteins generated by cells when surrounding by any type of stress.

For this reason, we have set up different protocols submitting cells to thermal shocks by extreme conditions of temperature and humidity (heat, cold, dry, wet), which allow us to determine the protective and functional capacity of different products and active ingredients.

  • Normal Human Epidermal Keratinocytes (NHEK).
  • Normal Human Dermal Fibroblasts (NHDF).
  • Normal Human Epidermal Melanocytes (NHEM).

 

 

Slimming Activity

We evaluate the slimming capacity of a specific ingredient or end product, through inhibition of fatty acid synthesis, termogenesis and lipolysis stimulation, lipogenesis inhibition, increase of transformation in brown adipose tissue, etc.

  • Human Pre-Adipocytes (HPAd).
  • Human Adipocytes (HAd).
  • Murine Pre-adipocytes (3T3-L1).

 

 

Epigenetics and Genomics

We analyze the epigenetic effects of a determined active or product, through quantification of the protective effects against the DNA methylation induced by an external agent, or by the analysis of the effects on the expression of microRNAs directly involved in skin health and quality.

On the other hand, we assess the effects on the expression of the whole human genome, through analysis by microarray. We obtain genes differentially expressed (threshold) and metabolic pathways affected.

 

  • Human cell lines (keratinocytes HaCaT and NHEK, fibroblasts NHDF, human follicle dermal papilla cells HFDPC).
  • Medaka eleutheroembryos.
  • 3D Reconstructed Human Skin (RHE).